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Gary Schroth, PhD presents an introduction to index hopping, clarifies how to minimize index hopping amounts by subsequent library prep best practices, and discusses how to eliminate index-hopped reads employing one of a kind dual indexing combos.
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The MiniSeq Swift produced a recipe transform that uses the grafted oligo for your i5 index priming, so it truly is browse from the ahead orientation comparable to a MiSeq.
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Produce entire-genome sequencing libraries and successfully interrogate samples with limited readily available DNA.
Established C Index Adapters Make reference to Index two (i5) Orientation To find out more regarding how to enter i5 bases on the sample sheet in forward or reverse enhance orientation.
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Special twin indexing provides totally exclusive, unrelated index sequences to each ends of every fragment of DNA all through library preparation.
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